Co-regulation of polar mRNA transport and lifespan in budding yeast Saccharomyces cerevisiae

Dmitri Taranukha, Arie Budovsky, Nikolai Gobshtis, Alex Braiman, Ziv Porat, Stella Aronov, Vadim E. Fraifeld

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Recent studies have uncovered the links between aging, rejuvenation and polar protein transport in the budding yeast Saccharomyces cerevisiae. Here, we examined a still unexplored possibility for co-regulation of polar mRNA transport and lifespan. To monitor the amount and distribution of mRNA-containing granules in mother and daughter cells, we used a fluorescent mRNA-labeling system, with MFA2 as a reporter gene. The results obtained showed that deletion of the selected longevity regulators in budding yeast had a significant impact on the polar mRNA transport. This included changes in the amount of mRNA-containing granules in cytoplasm, their aggregation and distribution between the mother and daughter cells. A significant negative correlation was found between strain-specific longevity, amount of granules and total fluorescent intensity both in mother and daughter cells. As indicated by the coefficient of determination, approximately 50-75% of variation in yeast lifespan could be attributed to the differences in polar mRNA transport.

Original languageEnglish
Pages (from-to)4275-4280
Number of pages6
JournalCell Cycle
Issue number22
StatePublished - 15 Nov 2012


  • Cell polarity
  • Granules
  • ImageStreamX
  • Lifespan
  • Longevity genes
  • P-bodies
  • Yeast
  • mRNA transport


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