Cloning of LL5, a novel protein encoding cDNA from a rat pituitary library

Linet Levi; Israel Hanukoglu; Moshe Raikhinstein; Fortune Kohen; Yitzhak Koch

doi:10.1016/0167-4781(93)90171-9

Cloning of LL5, a novel protein encoding cDNA from a rat pituitary library

Linet Levi
, Israel Hanukoglu
, Moshe Raikhinstein
, Fortune Kohen
, Yitzhak Koch

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

While screening a rat pituitary cDNA library for a peptide hormone receptor, we identified a cDNA that represents a novel gene. The 3.8 kb cDNA has an open reading frame of 2.3 kb encoding a protein of 781 amino acids (M_r = 87 507). Southern blot analysis of rat, mouse, bovine and human genomic DNAs revealed that a homologous gene is present in these species probably in a single copy. Northern blot analysis showed that in addition to the pituitary gland, the gene is also expressed in other rat tissues. Scanning of DNA and protein databanks revealed no significant homology to any other sequence. Thus, this gene encodes a heretofore unidentified protein.

Original language	English
Pages (from-to)	342-344
Number of pages	3
Journal	BBA - Gene Structure and Expression
Volume	1216
Issue number	2
DOIs	https://doi.org/10.1016/0167-4781(93)90171-9
State	Published - 16 Nov 1993
Externally published	Yes

Keywords

(Rat pituitary gland)
Anti-idiotypic antibody
Cloning
Gonadotropin-releasing hormone
Novel protein
Single copy gene
cDNA

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10.1016/0167-4781(93)90171-9

Cite this

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title = "Cloning of LL5, a novel protein encoding cDNA from a rat pituitary library",

abstract = "While screening a rat pituitary cDNA library for a peptide hormone receptor, we identified a cDNA that represents a novel gene. The 3.8 kb cDNA has an open reading frame of 2.3 kb encoding a protein of 781 amino acids (Mr = 87 507). Southern blot analysis of rat, mouse, bovine and human genomic DNAs revealed that a homologous gene is present in these species probably in a single copy. Northern blot analysis showed that in addition to the pituitary gland, the gene is also expressed in other rat tissues. Scanning of DNA and protein databanks revealed no significant homology to any other sequence. Thus, this gene encodes a heretofore unidentified protein.",

keywords = "(Rat pituitary gland), Anti-idiotypic antibody, Cloning, Gonadotropin-releasing hormone, Novel protein, Single copy gene, cDNA",

author = "Linet Levi and Israel Hanukoglu and Moshe Raikhinstein and Fortune Kohen and Yitzhak Koch",

note = "Funding Information: preparation of the anti-idiotypic antibodies. This research was supported in part by grants from The Center for Neurosciences and The Leo and Julia Forch-heimer Center for Molecular Genetics at the Weizmann Institute of Science.",

year = "1993",

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doi = "10.1016/0167-4781(93)90171-9",

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AU - Levi, Linet

AU - Hanukoglu, Israel

AU - Raikhinstein, Moshe

AU - Kohen, Fortune

AU - Koch, Yitzhak

N1 - Funding Information: preparation of the anti-idiotypic antibodies. This research was supported in part by grants from The Center for Neurosciences and The Leo and Julia Forch-heimer Center for Molecular Genetics at the Weizmann Institute of Science.

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N2 - While screening a rat pituitary cDNA library for a peptide hormone receptor, we identified a cDNA that represents a novel gene. The 3.8 kb cDNA has an open reading frame of 2.3 kb encoding a protein of 781 amino acids (Mr = 87 507). Southern blot analysis of rat, mouse, bovine and human genomic DNAs revealed that a homologous gene is present in these species probably in a single copy. Northern blot analysis showed that in addition to the pituitary gland, the gene is also expressed in other rat tissues. Scanning of DNA and protein databanks revealed no significant homology to any other sequence. Thus, this gene encodes a heretofore unidentified protein.

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KW - (Rat pituitary gland)

KW - Anti-idiotypic antibody

KW - Cloning

KW - Gonadotropin-releasing hormone

KW - Novel protein

KW - Single copy gene

KW - cDNA

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Cloning of LL5, a novel protein encoding cDNA from a rat pituitary library

Abstract

Keywords

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