TY - JOUR
T1 - Cloning of LL5, a novel protein encoding cDNA from a rat pituitary library
AU - Levi, Linet
AU - Hanukoglu, Israel
AU - Raikhinstein, Moshe
AU - Kohen, Fortune
AU - Koch, Yitzhak
N1 - Funding Information:
preparation of the anti-idiotypic antibodies. This research was supported in part by grants from The Center for Neurosciences and The Leo and Julia Forch-heimer Center for Molecular Genetics at the Weizmann Institute of Science.
PY - 1993/11/16
Y1 - 1993/11/16
N2 - While screening a rat pituitary cDNA library for a peptide hormone receptor, we identified a cDNA that represents a novel gene. The 3.8 kb cDNA has an open reading frame of 2.3 kb encoding a protein of 781 amino acids (Mr = 87 507). Southern blot analysis of rat, mouse, bovine and human genomic DNAs revealed that a homologous gene is present in these species probably in a single copy. Northern blot analysis showed that in addition to the pituitary gland, the gene is also expressed in other rat tissues. Scanning of DNA and protein databanks revealed no significant homology to any other sequence. Thus, this gene encodes a heretofore unidentified protein.
AB - While screening a rat pituitary cDNA library for a peptide hormone receptor, we identified a cDNA that represents a novel gene. The 3.8 kb cDNA has an open reading frame of 2.3 kb encoding a protein of 781 amino acids (Mr = 87 507). Southern blot analysis of rat, mouse, bovine and human genomic DNAs revealed that a homologous gene is present in these species probably in a single copy. Northern blot analysis showed that in addition to the pituitary gland, the gene is also expressed in other rat tissues. Scanning of DNA and protein databanks revealed no significant homology to any other sequence. Thus, this gene encodes a heretofore unidentified protein.
KW - (Rat pituitary gland)
KW - Anti-idiotypic antibody
KW - Cloning
KW - Gonadotropin-releasing hormone
KW - Novel protein
KW - Single copy gene
KW - cDNA
UR - http://www.scopus.com/inward/record.url?scp=0027504053&partnerID=8YFLogxK
U2 - 10.1016/0167-4781(93)90171-9
DO - 10.1016/0167-4781(93)90171-9
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C2 - 8241284
AN - SCOPUS:0027504053
SN - 0167-4781
VL - 1216
SP - 342
EP - 344
JO - BBA - Gene Structure and Expression
JF - BBA - Gene Structure and Expression
IS - 2
ER -