Characterization of winter dysentery bovine coronavirus isolated from cattle in israel

Dan David; Nick Storm; Waksman Ilan; Asaf Sol

doi:10.3390/v13061070

Characterization of winter dysentery bovine coronavirus isolated from cattle in israel

Dan David
, Nick Storm
, Waksman Ilan
, Asaf Sol

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Bovine coronavirus (BCoV) is the causative agent of winter dysentery (WD). In adult dairy cattle, WD is characterized by hemorrhagic diarrhea and a reduction in milk production. Therefore, WD leads to significant economic losses in dairy farms. In this study, we aimed to isolate and characterize local BCoV strains. BCoV positive samples, collected during 2017–2021, were used to amplify and sequence the S1 domain of S glycoprotein and the full hemagglutinin esterase gene. Based on our molecular analysis, local strains belong to different genetic variants circulating in dairy farms in Israel. Phylogenetic analysis revealed that all local strains clustered together and in proximity to other BCoV circulating in the area. Additionally, we found that local strains are genetically distant from the reference enteric strain Mebus. To our knowledge, this is the first report providing molecular data on BCoV circulating in Israel.

Original language	English
Article number	1070
Journal	Viruses
Volume	13
Issue number	6
DOIs	https://doi.org/10.3390/v13061070
State	Published - Jun 2021
Externally published	Yes

Keywords

Bovine coronavirus
Hemagglutinin esterase
S1
Spike
Winter dysentery

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10.3390/v13061070

Cite this

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title = "Characterization of winter dysentery bovine coronavirus isolated from cattle in israel",

abstract = "Bovine coronavirus (BCoV) is the causative agent of winter dysentery (WD). In adult dairy cattle, WD is characterized by hemorrhagic diarrhea and a reduction in milk production. Therefore, WD leads to significant economic losses in dairy farms. In this study, we aimed to isolate and characterize local BCoV strains. BCoV positive samples, collected during 2017{\textendash}2021, were used to amplify and sequence the S1 domain of S glycoprotein and the full hemagglutinin esterase gene. Based on our molecular analysis, local strains belong to different genetic variants circulating in dairy farms in Israel. Phylogenetic analysis revealed that all local strains clustered together and in proximity to other BCoV circulating in the area. Additionally, we found that local strains are genetically distant from the reference enteric strain Mebus. To our knowledge, this is the first report providing molecular data on BCoV circulating in Israel.",

keywords = "Bovine coronavirus, Hemagglutinin esterase, S1, Spike, Winter dysentery",

author = "Dan David and Nick Storm and Waksman Ilan and Asaf Sol",

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AU - David, Dan

AU - Storm, Nick

AU - Ilan, Waksman

AU - Sol, Asaf

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N2 - Bovine coronavirus (BCoV) is the causative agent of winter dysentery (WD). In adult dairy cattle, WD is characterized by hemorrhagic diarrhea and a reduction in milk production. Therefore, WD leads to significant economic losses in dairy farms. In this study, we aimed to isolate and characterize local BCoV strains. BCoV positive samples, collected during 2017–2021, were used to amplify and sequence the S1 domain of S glycoprotein and the full hemagglutinin esterase gene. Based on our molecular analysis, local strains belong to different genetic variants circulating in dairy farms in Israel. Phylogenetic analysis revealed that all local strains clustered together and in proximity to other BCoV circulating in the area. Additionally, we found that local strains are genetically distant from the reference enteric strain Mebus. To our knowledge, this is the first report providing molecular data on BCoV circulating in Israel.

AB - Bovine coronavirus (BCoV) is the causative agent of winter dysentery (WD). In adult dairy cattle, WD is characterized by hemorrhagic diarrhea and a reduction in milk production. Therefore, WD leads to significant economic losses in dairy farms. In this study, we aimed to isolate and characterize local BCoV strains. BCoV positive samples, collected during 2017–2021, were used to amplify and sequence the S1 domain of S glycoprotein and the full hemagglutinin esterase gene. Based on our molecular analysis, local strains belong to different genetic variants circulating in dairy farms in Israel. Phylogenetic analysis revealed that all local strains clustered together and in proximity to other BCoV circulating in the area. Additionally, we found that local strains are genetically distant from the reference enteric strain Mebus. To our knowledge, this is the first report providing molecular data on BCoV circulating in Israel.

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KW - Hemagglutinin esterase

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KW - Spike

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Characterization of winter dysentery bovine coronavirus isolated from cattle in israel

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Keywords

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