cDNA cloning and sequence analysis of the bovine adrenocorticotropic hormone (ACTH) receptor

Moshe Raikhinstein, Muriel Zohar, Israel Hanukoglu

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35 Scopus citations


We isolated five independent cDNAs of nearly 3000 bp for the bovine ACTH receptor by screening adrenal cortex cDNA libraries with a PCR cloned cDNA fragment. The deduced receptor sequence includes 297 residues (Mr = 33 258) with 81% identity with the human ACTH receptor, and shows seven hydrophobic transmembrane domains. The calculated Mr of the receptor is smaller than the 40-45 kDa observed in crosslinking studies with labeled ACTH. Since the bovine and human receptors have two glycosylation motifs in the N-terminus, the difference may result from glycosylation of the receptor. Analysis of the sequences of both bovine and human receptors revealed a single protein kinase A phosphorylation motif located in the third intraceullular loop (Ser-209) juxtaposed to a protein kinase C phosphorylation motif (Thr-204). Thus, the involvement of protein kinase A and C pathways in ACTH action may be mediated in part by phosphorylation of the ACTH receptor at these motifs. The 3′-untranslated region of the bovine cDNA is > 2000 bp and includes two inverse repeats giving an extensive and strong secondary structure to the ACTH receptor RNA.

Original languageEnglish
Pages (from-to)329-332
Number of pages4
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Issue number3
StatePublished - 17 Feb 1994
Externally publishedYes


  • ACTH receptor
  • Molecular cloning
  • Nucleotide sequence


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