Broadband visible light induced NO formation

Rachel Lubart, Maor Eichler, Harry Friedmann, N. Savion, Haim Breitbart, Rinat Ankri

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


Nitric oxide formation is a potential mechanism for photobiomodulation because it is synthesized in cells by nitric oxide synthase (NOS), which contains both flavin and heme, and thus absorbs visible light. The purpose of this work was to study broadband visible light induced NO formation in various cells. Cardiac, endothelial, sperm cells and RAW 264.7 macrophages were illuminated with broadband visible light, 40-130mW/cm2, 2.4-39J/cm2, and nitric oxide production was quantified by using the Griess reagent. The results showed that visible light illumination increased NO concentration both in sperm and endothelial cells, but not in cardiac cells. Activation of RAW 264.7 macrophages was very small. It thus appears that NO is involved in photobiomodulation, though different light parameters and illumination protocols are needed to induce NO in various cells.

Original languageEnglish
Title of host publicationLaser Florence 2008 - A Bridge to the Laser Medicine World
Subtitle of host publicationSelected Presentations at the International Laser Medicine Congress
Number of pages4
StatePublished - 2009
Externally publishedYes
EventInternational Laser Medicine Congress, Laser Florence 2008: A Bridge To The Laser Medicine World - Firenze, Italy
Duration: 31 Oct 20081 Nov 2008

Publication series

NameAIP Conference Proceedings
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616


ConferenceInternational Laser Medicine Congress, Laser Florence 2008: A Bridge To The Laser Medicine World


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