@inbook{a62f625316364f16b65c46f2c3a5ad59,
title = "Blotting from PhastGel to membranes by ultrasound",
abstract = "Ultrasound based approach for enhanced protein blotting is proposed. Three minutes of ultrasound exposure (1 MHz, 2.5 W/cm2) was suffi cient for a clear transfer of proteins from a polyacrylamide gel (PhastGel) to nitrocellulose or Nylon 66 Biotrans membrane. The proteins evaluated were prestained sodium dodecyl sulfate-polyacrylamide standards (18,500–106,000 Da) and 14C-labeled Rainbow protein molecular weight markers (14,300–200,000 Da).",
keywords = "Mass transfer, Nitrocellulose membranes, Nylon 66 biotrans membranes, PhastGel, Polyacrylamide gel, Protein blotting, Ultrasound",
author = "Joseph Kost and Aharon Azagury",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2015.",
year = "2015",
doi = "10.1007/978-1-4939-2694-7\_25",
language = "אנגלית",
isbn = "978-1-4939-2693-0",
volume = "1312",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "237--246",
booktitle = "Western Blotting Methods and Protocols",
}