Blotting from PhastGel to membranes by ultrasound

Joseph Kost, Aharon Azagury

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Ultrasound based approach for enhanced protein blotting is proposed. Three minutes of ultrasound exposure (1 MHz, 2.5 W/cm2) was suffi cient for a clear transfer of proteins from a polyacrylamide gel (PhastGel) to nitrocellulose or Nylon 66 Biotrans membrane. The proteins evaluated were prestained sodium dodecyl sulfate-polyacrylamide standards (18,500–106,000 Da) and 14C-labeled Rainbow protein molecular weight markers (14,300–200,000 Da).

Original languageEnglish
Pages (from-to)237-246
Number of pages10
JournalMethods in Molecular Biology
StatePublished - 2015
Externally publishedYes


  • Mass transfer
  • Nitrocellulose membranes
  • Nylon 66 biotrans membranes
  • PhastGel
  • Polyacrylamide gel
  • Protein blotting
  • Ultrasound


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