TY - JOUR
T1 - Biochar mediates systemic response of strawberry to foliar fungal pathogens
AU - Harel, Yael Meller
AU - Elad, Yigal
AU - Rav-David, Dalia
AU - Borenstein, Menachem
AU - Shulchani, Ran
AU - Lew, Beni
AU - Graber, Ellen R.
N1 - Funding Information:
Acknowledgements The authors wish to thank Dr. Nir Dai and Dr. David Ezra for their help with the molecular work. This work was supported by grants from The Autonomous Province of Trento, Call for Proposal Major Projects 2006, Project ENVIROCHANGE and by the Chief Scientist of the Ministry of Agriculture and Rural Development of Israel, project number 301-0693-10. This paper is contribution no. 506/11 of the Agricultural Research Organization, The Volcani Center, Israel.
PY - 2012/8
Y1 - 2012/8
N2 - Background and Aims: Various biochars added to soil have been shown to improve plant performance. Moreover, a wood biochar was found to induce tomato and pepper plant systemic resistance to two foliar fungal pathogens. The aim of this study was to explore the ability of wood biochar and greenhouse waste biochar to induce systemic resistance in strawberry plants against Botrytis cinerea, Colletotrichum acutatum and Podosphaera apahanis, and to examine at the molecular level some of their impacts on plant defense mechanisms. Methods: Disease development tests on plants grown on 1 or 3% biochar-amended potting mixture, and quantification of relative expression of 5 plant defense-related genes (FaPR1, Faolp2, Fra a3, Falox, and FaWRKY1) by real-time PCR were carried out. Results: Biochar addition to the potting medium of strawberry plants suppressed diseases caused by the three fungi, which have very different infection strategies. This suggests that biochar stimulated a range of general defense pathways, as confirmed by results of qPCR study of defense-related gene expression. Furthermore, primed-state of defense-related gene expression was observed upon infection by B. cinerea and P. aphanis. Conclusion: The ability of biochar amendment to promote transcriptional changes along different plant defense pathways probably contributes to its broad spectrum capacity for disease suppression.
AB - Background and Aims: Various biochars added to soil have been shown to improve plant performance. Moreover, a wood biochar was found to induce tomato and pepper plant systemic resistance to two foliar fungal pathogens. The aim of this study was to explore the ability of wood biochar and greenhouse waste biochar to induce systemic resistance in strawberry plants against Botrytis cinerea, Colletotrichum acutatum and Podosphaera apahanis, and to examine at the molecular level some of their impacts on plant defense mechanisms. Methods: Disease development tests on plants grown on 1 or 3% biochar-amended potting mixture, and quantification of relative expression of 5 plant defense-related genes (FaPR1, Faolp2, Fra a3, Falox, and FaWRKY1) by real-time PCR were carried out. Results: Biochar addition to the potting medium of strawberry plants suppressed diseases caused by the three fungi, which have very different infection strategies. This suggests that biochar stimulated a range of general defense pathways, as confirmed by results of qPCR study of defense-related gene expression. Furthermore, primed-state of defense-related gene expression was observed upon infection by B. cinerea and P. aphanis. Conclusion: The ability of biochar amendment to promote transcriptional changes along different plant defense pathways probably contributes to its broad spectrum capacity for disease suppression.
KW - Biotic stress
KW - Induced systemic resistance
KW - Plant disease
KW - Priming
KW - Systemic acquired resistance
KW - Systemic resistance
UR - http://www.scopus.com/inward/record.url?scp=84864145242&partnerID=8YFLogxK
U2 - 10.1007/s11104-012-1129-3
DO - 10.1007/s11104-012-1129-3
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AN - SCOPUS:84864145242
SN - 0032-079X
VL - 357
SP - 245
EP - 257
JO - Plant and Soil
JF - Plant and Soil
IS - 1
ER -