TY - JOUR
T1 - Assessment and detection
T2 - The effect of hair pigment on the incorporation of fatty acid ethyl esters (FAEE)
AU - Kulaga, Vivian
AU - Velazquez-Armenta, Yadira
AU - Aleksa, Katarina
AU - Vergee, Zulfikarali
AU - Koren, Gideon
N1 - Funding Information:
Acknowledgements — The authors acknowledge support received from the Canadian Institute for Health and Research. Gideon Koren is the holder of the Ivey Chair in Molecular Toxicology, University of Western Ontario.
PY - 2009
Y1 - 2009
N2 - Aims: The objective of the current study was to determine whether FAEE incorporation is affected by hair pigmentation. Methods: Black hooded LE rats were injected intraperitoneally daily with ethanol. Prior to dosing, black and white patches of fur were shaved and analyzed for baseline levels of FAEE using an adapted extraction procedure and GCMS method. Once the shaved 'patches' had grown back they were re-sampled along with hair outside the 'patches', referred to as 'no patch' hair, and tested for post-treatment FAEE levels in the same manner. Blood was also sampled for pharmacokinetic analysis of ethanol. Results: Total FAEE levels were significantly higher in post-treatment hair (black and white) compared to baseline (pre-treatment) levels. Total FAEE levels were also significantly higher in post-treatment 'patch' hair (black and white) compared to 'no patch' hair. No significant differences were found between post-treatment black and white hair. The FAEE profiles were similar between black and white hair, with FAEE levels being highest for ethyl myristate, followed by ethyl stearate, palmitate, and then oleate. Conclusion: FAEE incorporation into hair does not appear to be affected by hair pigment, which is in congruence with what is known about the chemistry of drug-melanin interactions. This is important in avoiding potential bias and discrimination in the interpretation of alcohol abuse based on hair color.
AB - Aims: The objective of the current study was to determine whether FAEE incorporation is affected by hair pigmentation. Methods: Black hooded LE rats were injected intraperitoneally daily with ethanol. Prior to dosing, black and white patches of fur were shaved and analyzed for baseline levels of FAEE using an adapted extraction procedure and GCMS method. Once the shaved 'patches' had grown back they were re-sampled along with hair outside the 'patches', referred to as 'no patch' hair, and tested for post-treatment FAEE levels in the same manner. Blood was also sampled for pharmacokinetic analysis of ethanol. Results: Total FAEE levels were significantly higher in post-treatment hair (black and white) compared to baseline (pre-treatment) levels. Total FAEE levels were also significantly higher in post-treatment 'patch' hair (black and white) compared to 'no patch' hair. No significant differences were found between post-treatment black and white hair. The FAEE profiles were similar between black and white hair, with FAEE levels being highest for ethyl myristate, followed by ethyl stearate, palmitate, and then oleate. Conclusion: FAEE incorporation into hair does not appear to be affected by hair pigment, which is in congruence with what is known about the chemistry of drug-melanin interactions. This is important in avoiding potential bias and discrimination in the interpretation of alcohol abuse based on hair color.
UR - http://www.scopus.com/inward/record.url?scp=65549111345&partnerID=8YFLogxK
U2 - 10.1093/alcalc/agn114
DO - 10.1093/alcalc/agn114
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C2 - 19151159
AN - SCOPUS:65549111345
SN - 0735-0414
VL - 44
SP - 287
EP - 292
JO - Alcohol and Alcoholism
JF - Alcohol and Alcoholism
IS - 3
ER -