TY - JOUR
T1 - Antioxidant properties of bucillamine
T2 - Possible mode of action
AU - Mazor, Dalia
AU - Greenberg, Liya
AU - Shamir, Dror
AU - Meyerstein, Dan
AU - Meyerstein, Naomi
N1 - Funding Information:
This study was supported in part by a Grant from the Israel Science Foundation.
PY - 2006/10/27
Y1 - 2006/10/27
N2 - The antioxidant properties of Bucillamine (BUC), a di-thiol compound used for treatment of rheumatoid arthritis (RA) and its possible mode of action, were investigated. BUC exhibits potent antioxidant activity similar to those of trolox and ascorbic acid. It reduces the stable free radical diphenyl-2-picrylhydrazyl (DPPH) with IC50 of 18.5 ± 0.1 μmol, its relative antioxidant activity by the ferric reducing ability (FRAP) is 2.07 ± 0.01 mM and by the trolox equivalent antioxidant capacity (TEAC), 1.46 ± 0.05 mM. However, its superoxide and apparent hydroxyl radical scavenging activities are low (IC50 at millimolar concentrations). We found that BUC is a strong iron (II) and copper (II) chelator. This finding is very important since these metal ions are significantly higher in RA patients and may be involved in oxidative stress-induced damage. Our study suggests that BUC is a potent antioxidant which exerts its beneficial therapeutic activities in RA patients by metal chelation rather than by scavenging free radical species.
AB - The antioxidant properties of Bucillamine (BUC), a di-thiol compound used for treatment of rheumatoid arthritis (RA) and its possible mode of action, were investigated. BUC exhibits potent antioxidant activity similar to those of trolox and ascorbic acid. It reduces the stable free radical diphenyl-2-picrylhydrazyl (DPPH) with IC50 of 18.5 ± 0.1 μmol, its relative antioxidant activity by the ferric reducing ability (FRAP) is 2.07 ± 0.01 mM and by the trolox equivalent antioxidant capacity (TEAC), 1.46 ± 0.05 mM. However, its superoxide and apparent hydroxyl radical scavenging activities are low (IC50 at millimolar concentrations). We found that BUC is a strong iron (II) and copper (II) chelator. This finding is very important since these metal ions are significantly higher in RA patients and may be involved in oxidative stress-induced damage. Our study suggests that BUC is a potent antioxidant which exerts its beneficial therapeutic activities in RA patients by metal chelation rather than by scavenging free radical species.
KW - Antioxidant activity
KW - Bucillamine
KW - Metal chelation
KW - Reactive oxygen species
KW - Thiols
KW - Trolox
UR - http://www.scopus.com/inward/record.url?scp=33748599868&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2006.08.155
DO - 10.1016/j.bbrc.2006.08.155
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C2 - 16970913
AN - SCOPUS:33748599868
SN - 0006-291X
VL - 349
SP - 1171
EP - 1175
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -