TY - JOUR
T1 - Anti-inflammatory effects of Sarcopoterium spinosum extract
AU - Rozenberg, Konstantin
AU - Wollman, Ayala
AU - Ben-Shachar, Michaella
AU - Argaev-Frenkel, Lital
AU - Rosenzweig, Tovit
N1 - Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2020/3/1
Y1 - 2020/3/1
N2 - Ethnopharmacological relevance: Sarcopoterium spinosum is a Mediterranean plant, widely used by the Beduin traditional medicine. While its major use is for the treatment of diabetes, there are also documentations for its use as anti-inflammatory agent. This therapeutic potential of Sarcopoterium spinosum was not validated before. Aim of the study: To investigate the anti-inflammatory properties of Sarcopoterium spinosum extract (SSE). Materials and methods: Experiments were performed on RAW264.7 macrophages and bone marrow-derived macrophages (BMDM) and the effect of SSE on markers of inflammation was investigated. In addition, the effect of SSE on the development of inflammation in adipose-tissue of obese, insulin resistant mice was measured in KK-Ay mice and high fat diet (HFD)-fed mice. Results: Lipopolysaccharide (LPS) and SSE increased the viability of RAW264.7. In addition, the cells acquired distinct dendritic-like morphology, however, while LPS induced NO production and the mRNA expression of pro-inflammatory cytokines, SSE increased the mRNA expression of anti-inflammatory genes and blocked LPS effects. All three pathways of MAPK were activated by LPS and SSE, as demonstrated by the phosphorylation of ERK, p38 and JNK. NFκB was activated and Akt was phosphorylated by LPS, while SSE blocked this effects. STAT proteins were differently phosphorylated by SSE and LPS. Immunomodulatory effects of SSE were also found in BMDM. In adipose tissue of SSE-treated mice, less crown-like structures were found, and lower expression of pro-inflammatory adipocytokines was observed, although adipocytes hypertrophy was not affected. Conclusions: SSE has an immunomodulatory effects that affect macrophage function, and reduces adipose tissue inflammation. Identifying active component and clarifying its mechanism of action might support the development of new anti-inflammatory agent.
AB - Ethnopharmacological relevance: Sarcopoterium spinosum is a Mediterranean plant, widely used by the Beduin traditional medicine. While its major use is for the treatment of diabetes, there are also documentations for its use as anti-inflammatory agent. This therapeutic potential of Sarcopoterium spinosum was not validated before. Aim of the study: To investigate the anti-inflammatory properties of Sarcopoterium spinosum extract (SSE). Materials and methods: Experiments were performed on RAW264.7 macrophages and bone marrow-derived macrophages (BMDM) and the effect of SSE on markers of inflammation was investigated. In addition, the effect of SSE on the development of inflammation in adipose-tissue of obese, insulin resistant mice was measured in KK-Ay mice and high fat diet (HFD)-fed mice. Results: Lipopolysaccharide (LPS) and SSE increased the viability of RAW264.7. In addition, the cells acquired distinct dendritic-like morphology, however, while LPS induced NO production and the mRNA expression of pro-inflammatory cytokines, SSE increased the mRNA expression of anti-inflammatory genes and blocked LPS effects. All three pathways of MAPK were activated by LPS and SSE, as demonstrated by the phosphorylation of ERK, p38 and JNK. NFκB was activated and Akt was phosphorylated by LPS, while SSE blocked this effects. STAT proteins were differently phosphorylated by SSE and LPS. Immunomodulatory effects of SSE were also found in BMDM. In adipose tissue of SSE-treated mice, less crown-like structures were found, and lower expression of pro-inflammatory adipocytokines was observed, although adipocytes hypertrophy was not affected. Conclusions: SSE has an immunomodulatory effects that affect macrophage function, and reduces adipose tissue inflammation. Identifying active component and clarifying its mechanism of action might support the development of new anti-inflammatory agent.
KW - Adipose tissue inflammation
KW - Inflammation
KW - Macrophage
KW - Sarcopoterium spinosum
UR - http://www.scopus.com/inward/record.url?scp=85075484794&partnerID=8YFLogxK
U2 - 10.1016/j.jep.2019.112391
DO - 10.1016/j.jep.2019.112391
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C2 - 31730890
AN - SCOPUS:85075484794
SN - 0378-8741
VL - 249
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
M1 - 112391
ER -