Abstract
Structural age-related changes in cholinergic regions within the central nervous system of CW1 mice have been described previously. Since elevated calcium concentration has been suggested to play a role in the brain aging processes, the possible involvement of voltage dependent calcium channels in this degeneration was investigated. The binding characteristics of the calcium channel antagonist [3H]( + )PN 200-110 to brains of aged CW1 mice were studied. This ligand exhibited high affinity binding (Kd values in the range of 50–70 pM) to a single type of sites with a density (Bmax) of 150–200 fmol/mg protein. No significant differences were observed between the binding parameters measured in young (3 months), mid-aged (9 and 15 months) and old (20 months) mice. Autoradiographic study confirmed these results and extended them to specific brain regions. It is concluded that age-dependent degeneration processes, observed histologically in specific brain regions of these mice, are probably not related to alterations in voltage-dependent calcium channels.
| Original language | English |
|---|---|
| Pages (from-to) | 109-118 |
| Number of pages | 10 |
| Journal | Journal of Basic and Clinical Physiology and Pharmacology |
| Volume | 3 |
| Issue number | 2 |
| DOIs | |
| State | Published - Apr 1992 |
| Externally published | Yes |
Keywords
- [3H]( + )PN 200-110
- calcium antagonists
- central nervous system
- hippocampus