A shift in glycerolipid metabolism defines the follicular fluid of ivf patients with unexplained infertility

Albert Batushansky, Anish Zacharia, Alaa Shehadeh, Reut Bruck-Haimson, Daniel Saidemberg, Natalya M. Kogan, Chanchal Thomas Mannully, Shmuel Herzberg, Assaf Ben-Meir, Arieh Moussaieff

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Follicular fluid (FF) constitutes the microenvironment of the developing oocyte. We recently characterized its lipid composition and found lipid signatures of positive pregnancy outcome after in vitro fertilization (IVF). In the current study, we aimed to test the hypothesis that unexplained female infertility is related to lipid metabolism, given the lipid signature of positive-outcome IVF patients we previously found. Assuming that FF samples from IVF patients with male factor infertility can represent a non-hindered metabolic microenvironment, we compared them to FF taken from women with unexplained infertility. FF from patients undergoing IVF was examined for its lipid composition. We found highly increased triacylglycerol levels, with a lower abundance of monoacylglycerols, phospholipids and sphingolipids in the FF of patients with unexplained infertility. The alterations in the lipid class accumulation were independent of the body mass index (BMI) and were altogether kept across the age groups. Potential lipid biomarkers for pregnancy outcomes showed a highly discriminative abundance in the FF of unexplained infertility patients. Lipid abundance distinguished IVF patients with unrecognized infertility and provided a potential means for the evaluation of female fertility.

Original languageEnglish
Article number1135
Pages (from-to)1-13
Number of pages13
Issue number8
StatePublished - Aug 2020
Externally publishedYes


  • Follicular fluid
  • In vitro fertilization (IVF)
  • Lipidomics
  • Monoacylglycerols
  • Phospholipids
  • Triacylglycerols
  • Unexplained infertility


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