A general approach for antibody purification utilizing [Protein A-Catechol:Fe3+] macro-complexes

Guy Patchornik; Amnon Albeck

doi:10.1021/bc050361+

A general approach for antibody purification utilizing [Protein A-Catechol:Fe³⁺] macro-complexes

Guy Patchornik, Amnon Albeck

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A general platform for antibody purification utilizing free nonimmobilized Protein A modified with the strong metal chelator catechol (ProA-CAT) and Fe³⁺ ions is presented. The mechanism of purification requires formation and precipitation of macro-complexes composed of [ProA-CAT:IgG:Fe ³⁺]. Target IgGs are eluted directly from the precipitates (i.e. pellets) at pH 3 in high yields (71-80%) and high purity (>95%), without dissociating the [ProA-CAT:Fe³⁺] insoluble macro-complex.

Original language	English
Pages (from-to)	258-260
Number of pages	3
Journal	Bioconjugate Chemistry
Volume	17
Issue number	2
DOIs	https://doi.org/10.1021/bc050361+
State	Published - 2006
Externally published	Yes

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A general approach for antibody purification utilizing [Protein A-Catechol:Fe³⁺] macro-complexes

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