@inbook{46726968c7bc4f0995703e7456b17214,
title = "Using a Modified Proximity Ligation Protocol to Study the Interaction Between Chaperones and Associated Proteins",
abstract = "Molecular chaperones can interact with multiple proteins to form large networks. Understanding these interactions may shed light on the complexity of the chaperone functions. Here we developed a protocol for a modified proximity ligation-based methodology (PLA) for the detection of protein–protein interactions in order to understand how the Hsp70-Bag3 complex interacts with components of the Hippo signaling pathway. These experiments helped to elucidate the mechanisms of transmission of the proteotoxic stress signal to the Hippo pathway. The modified PLA technology has many advantages compared to co-immunoprecipitation protocols. It has higher sensitivity, is quantitative, and can be done in a 96-well format.",
keywords = "Bag3, Hippo pathway, Hsp70, LATS1/2, Protein complex, Proximity ligation assay, Yap",
author = "Simone Baldan and Meriin, \{Anatoli B.\} and Sherman, \{Michael Y.\}",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-3342-7\_13",
language = "אנגלית",
series = "Methods in Molecular Biology",
pages = "163--174",
booktitle = "Methods in Molecular Biology",
}