TY - JOUR
T1 - Towards rational chemosensor design through improved understanding of experimental parameter variation and tolerance in cyclodextrin-promoted fluorescence detection
AU - DiScenza, Dana J.
AU - Culton, Ella
AU - Verderame, Molly
AU - Lynch, Julie
AU - Serio, Nicole
AU - Levine, Mindy
N1 - Publisher Copyright:
© 2017 by the authors.
PY - 2017/12/1
Y1 - 2017/12/1
N2 - We have previously developed a highly efficient fluorescence-based toxicant-detection method that operates in complex environments to detect aromatic toxicants and toxicant metabolites with high sensitivity and selectivity. This method relies on the ability of γ-cyclodextrin to act as a supramolecular scaffold, and uses a variety of non-covalent interactions between the cyclodextrin, toxicant, and fluorophore to enable efficient detection. Reported herein is an investigation of the effect of various experimental parameters, including host concentration, temperature, pH, salt, and solvent, on the observed energy-transfer efficiencies. These results advance our understanding of γ-cyclodextrin-based association complexes and provide crucial information for the development of fluorescence-based sensors using such complexation and the resultant fluorescence-based detection.
AB - We have previously developed a highly efficient fluorescence-based toxicant-detection method that operates in complex environments to detect aromatic toxicants and toxicant metabolites with high sensitivity and selectivity. This method relies on the ability of γ-cyclodextrin to act as a supramolecular scaffold, and uses a variety of non-covalent interactions between the cyclodextrin, toxicant, and fluorophore to enable efficient detection. Reported herein is an investigation of the effect of various experimental parameters, including host concentration, temperature, pH, salt, and solvent, on the observed energy-transfer efficiencies. These results advance our understanding of γ-cyclodextrin-based association complexes and provide crucial information for the development of fluorescence-based sensors using such complexation and the resultant fluorescence-based detection.
KW - Cyclodextrin
KW - Energy transfer
KW - Fluorescence detection
KW - Linear discriminant analysis
KW - Optical spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=85045390398&partnerID=8YFLogxK
U2 - 10.3390/chemosensors5040034
DO - 10.3390/chemosensors5040034
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AN - SCOPUS:85045390398
SN - 2227-9040
VL - 5
JO - Chemosensors
JF - Chemosensors
IS - 4
M1 - 34
ER -