TY - JOUR
T1 - Near-infrared, dual-ratiometric fluorescent label for measurement of pH
AU - Povrozin, Yevgen A.
AU - Markova, Larysa I.
AU - Tatarets, Anatoliy L.
AU - Sidorov, Vadim I.
AU - Terpetschnig, Ewald A.
AU - Patsenker, Leonid D.
PY - 2009/7/15
Y1 - 2009/7/15
N2 - We describe the spectral properties of an amine-reactive, pH-sensitive, long-wavelength ratiometric fluorescent label having a pKa in the physiological pH range. The label exhibits its main absorption and emission in the near-infrared (NIR) region. On deprotonation, a blue shift of the excitation maximum is observed. Importantly, both the protonated and deprotonated forms of the label are fluorescent, with the deprotonated form having an extremely large Stokes shift of more than 100 nm. The spectral and photophysical properties of this pH label are compared with the properties of the protein-conjugated forms. Due to the observed pKa shift to the acidic pH range upon conjugation to proteins, such labels are ideal for studying phagocytic events and their regulation by drugs and/or environmental factors.
AB - We describe the spectral properties of an amine-reactive, pH-sensitive, long-wavelength ratiometric fluorescent label having a pKa in the physiological pH range. The label exhibits its main absorption and emission in the near-infrared (NIR) region. On deprotonation, a blue shift of the excitation maximum is observed. Importantly, both the protonated and deprotonated forms of the label are fluorescent, with the deprotonated form having an extremely large Stokes shift of more than 100 nm. The spectral and photophysical properties of this pH label are compared with the properties of the protein-conjugated forms. Due to the observed pKa shift to the acidic pH range upon conjugation to proteins, such labels are ideal for studying phagocytic events and their regulation by drugs and/or environmental factors.
KW - Fluorescence lifetime
KW - NIR fluorescent probe
KW - Ratiometric pH measurement
UR - http://www.scopus.com/inward/record.url?scp=65849265264&partnerID=8YFLogxK
U2 - 10.1016/j.ab.2009.03.047
DO - 10.1016/j.ab.2009.03.047
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C2 - 19351524
AN - SCOPUS:65849265264
SN - 0003-2697
VL - 390
SP - 136
EP - 140
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -