Milk-specific antibody measurement by ELISA: development of an assay

M. A. Firer, C. S. Hosking, D. J. Hill

نتاج البحث: نشر في مجلةمقالةمراجعة النظراء

11 اقتباسات (Scopus)

ملخص

This paper describes the development of a simple, sensitive and specific enzyme-linked immunosorbent assay (ELISA) for the measurement of serum IgG, IgA and IgM anti-bodies to cow's milk protein. The specificity of this assay was determined by preparing immunosorbents of cow's milk and human serum albumin (HSA). Fractionation of the antiserum-enzyme label by gel filtration and affinity chromatography showed that the conjugate preparation contained moieties of antiserum-enzyme, and antiserum-enzyme polymers. The preparation also contained enzyme-enzyme and antiserum-antiserum conjugates. Purification of the labelled reagent did not improve the sensitivity. The reagents and conditions used have been analyzed for purposes of standardisation.

اللغة الأصليةالإنجليزيّة
الصفحات (من إلى)31-40
عدد الصفحات10
دوريةJournal of Immunological Methods
مستوى الصوت46
رقم الإصدار1
المعرِّفات الرقمية للأشياء
حالة النشرنُشِر - 15 أكتوبر 1981
منشور خارجيًانعم

بصمة

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