Effect of subinhibitory concentrations of antibiotics on extracellular shiga-like toxin I

J. N. Walterspiel, Ardythe L. Morrow, T. G. Cleary, S. Ashkenazi

نتاج البحث: نشر في مجلةمقالةمراجعة النظراء

176 اقتباسات (Scopus)

ملخص

Patients with diarrhea due to strains of enterohemorrhagic Escherichia coli (EHEC) (e. g. O157:H7) might be at a higher risk of developing hemolytic uremic syndrome when treated with antimicrobial agents. It has been suggested that this might be due to an increase of release or production of vero or shiga-like toxin from such organisms, possibly as a stress response to antimicrobial agents. The aim of this study was to detect such increases in extracellular toxin in vitro with a newly developed method that exposed EHEC to high sublethal concentrations followed by a recovery phase at progressively lower concentrations. Five strains of EHEC were exposed to continuously changing concentrations of ciprofloxacin, co-trimoxazole, cefixime and tetracycline. The amount of free shiga-like toxin I (SLT-I) released was compared to the amount released from inocula that were not exposed to antibiotics. There were significant differences between the five EHEC strains in the amount of toxin detected after exposure to antimicrobial agents (p<0.001). Equally important was the type of antibiotic (p<0.001), with ciprofloxacin inducing the largest increase ranging from 169 to 436%, followed by co-trimoxazole, cefixime and tetracycline. In addition, the increases in free toxin correlated with the concentration of the antibiotics (p<0.001). The association between antibiotic-induced increases in SLT-I produced by strains of EHEC and certain classes of antibiotics might influence the analysis of future epidemiological studies on risk factors for HUS.

اللغة الأصليةالإنجليزيّة
الصفحات (من إلى)25-29
عدد الصفحات5
دوريةInfection
مستوى الصوت20
رقم الإصدار1
المعرِّفات الرقمية للأشياء
حالة النشرنُشِر - يناير 1992
منشور خارجيًانعم

بصمة

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