TY - JOUR
T1 - Detection and quantification of fatty acid ethyl esters in meconium by headspace-solid-phase microextraction and gas chromatography-mass spectrometry
AU - Hutson, J. R.
AU - Aleksa, K.
AU - Pragst, F.
AU - Koren, G.
N1 - Funding Information:
JRH is supported by the University of Toronto’s Comprehensive Research Experience for Medical Students Summer Studentship and a CIHR MD/PhD Studentship. GK is the holder of the Ivey Chair in Molecular Toxicology, The Department of Medicine, University of Western Ontario.
PY - 2009/1/1
Y1 - 2009/1/1
N2 - Meconium fatty acid ethyl esters (FAEEs) are currently used as biomarkers to detect heavy prenatal alcohol exposure. We introduce a novel technique to quantify FAEEs in meconium using headspace-solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). This method improves on previous approaches by decreasing sample preparation time, eliminating the need for organic solvents, and reducing the required sample size. Using 50 mg of meconium, the detection limits of FAEEs ranged from 0.05 to 0.16 nmol/g and had good reproducibility making it ideal for routine analysis of clinical samples.
AB - Meconium fatty acid ethyl esters (FAEEs) are currently used as biomarkers to detect heavy prenatal alcohol exposure. We introduce a novel technique to quantify FAEEs in meconium using headspace-solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). This method improves on previous approaches by decreasing sample preparation time, eliminating the need for organic solvents, and reducing the required sample size. Using 50 mg of meconium, the detection limits of FAEEs ranged from 0.05 to 0.16 nmol/g and had good reproducibility making it ideal for routine analysis of clinical samples.
KW - Biomarker
KW - Fatty acid ethyl esters
KW - Gas chromatography
KW - Headspace-solid-phase microextraction
KW - Meconium
UR - https://www.scopus.com/pages/publications/57549116363
U2 - 10.1016/j.jchromb.2008.10.056
DO - 10.1016/j.jchromb.2008.10.056
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 19056326
AN - SCOPUS:57549116363
SN - 1570-0232
VL - 877
SP - 8
EP - 12
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 1-2
ER -