TY - JOUR
T1 - Collagen-cellulose composite thin films that mimic soft-tissue and allow stem-cell orientation
AU - Steele, Terry W.J.
AU - Huang, Charlotte L.
AU - Nguyen, Evelyne
AU - Sarig, Udi
AU - Kumar, Saranya
AU - Widjaja, Effendi
AU - Loo, Joachim S.C.
AU - Machluf, Marcelle
AU - Boey, Freddy
AU - Vukadinovic, Zlata
AU - Hilfiker, Andreas
AU - Venkatraman, Subbu S.
N1 - Funding Information:
Acknowledgments The authors acknowledge and appreciate the help and support rendered by Final Year Project Student, Wang Qianying Dione. This research was most generously supported by the Singapore National Research Foundation under parts of the following programs: NRF2010NRF-POC002-019, NRF 2007 NRF-CRP 002-12, and the CREATE program: The Regenerative Medicine Initiative in Cardiac Restoration Therapy Research.
PY - 2013/8
Y1 - 2013/8
N2 - Mechanical properties of collagen films are less than ideal for biomaterial development towards musculoskeletal repair or cardiovascular applications. Herein, we present a collagen-cellulose composite film (CCCF) compared against swine small intestine submucosa in regards to mechanical properties, cell growth, and histological analysis. CCCF was additionally characterized by FE-SEM, NMR, mass spectrometry, and Raman Microscopy to elucidate its physical structure, collagen-cellulose composition, and structure activity relationships. Mechanical properties of the CCCF were tested in both wet and dry environments, with anisotropic stress-strain curves that mimicked soft-tissue. Mesenchymal stem cells, human umbilical vein endothelial cells, and human coronary artery smooth muscle cells were able to proliferate on the collagen films with specific cell orientation. Mesenchymal stem cells had a higher proliferation index and were able to infiltrate CCCF to a higher degree than small intestine submucosa. With the underlying biological properties, we present a collagen-cellulose composite film towards forthcoming biomaterial-related applications. Graphical Abstract: [Figure not available: see fulltext.]
AB - Mechanical properties of collagen films are less than ideal for biomaterial development towards musculoskeletal repair or cardiovascular applications. Herein, we present a collagen-cellulose composite film (CCCF) compared against swine small intestine submucosa in regards to mechanical properties, cell growth, and histological analysis. CCCF was additionally characterized by FE-SEM, NMR, mass spectrometry, and Raman Microscopy to elucidate its physical structure, collagen-cellulose composition, and structure activity relationships. Mechanical properties of the CCCF were tested in both wet and dry environments, with anisotropic stress-strain curves that mimicked soft-tissue. Mesenchymal stem cells, human umbilical vein endothelial cells, and human coronary artery smooth muscle cells were able to proliferate on the collagen films with specific cell orientation. Mesenchymal stem cells had a higher proliferation index and were able to infiltrate CCCF to a higher degree than small intestine submucosa. With the underlying biological properties, we present a collagen-cellulose composite film towards forthcoming biomaterial-related applications. Graphical Abstract: [Figure not available: see fulltext.]
UR - https://www.scopus.com/pages/publications/84880848598
U2 - 10.1007/s10856-013-4940-3
DO - 10.1007/s10856-013-4940-3
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C2 - 23670603
AN - SCOPUS:84880848598
SN - 0957-4530
VL - 24
SP - 2013
EP - 2027
JO - Journal of Materials Science: Materials in Medicine
JF - Journal of Materials Science: Materials in Medicine
IS - 8
ER -