TY - JOUR
T1 - Blotting from PhastGel to membranes by ultrasound
AU - Kost, Joseph
AU - Azagury, Aharon
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2015.
PY - 2015
Y1 - 2015
N2 - Ultrasound based approach for enhanced protein blotting is proposed. Three minutes of ultrasound exposure (1 MHz, 2.5 W/cm2) was suffi cient for a clear transfer of proteins from a polyacrylamide gel (PhastGel) to nitrocellulose or Nylon 66 Biotrans membrane. The proteins evaluated were prestained sodium dodecyl sulfate-polyacrylamide standards (18,500–106,000 Da) and 14C-labeled Rainbow protein molecular weight markers (14,300–200,000 Da).
AB - Ultrasound based approach for enhanced protein blotting is proposed. Three minutes of ultrasound exposure (1 MHz, 2.5 W/cm2) was suffi cient for a clear transfer of proteins from a polyacrylamide gel (PhastGel) to nitrocellulose or Nylon 66 Biotrans membrane. The proteins evaluated were prestained sodium dodecyl sulfate-polyacrylamide standards (18,500–106,000 Da) and 14C-labeled Rainbow protein molecular weight markers (14,300–200,000 Da).
KW - Mass transfer
KW - Nitrocellulose membranes
KW - Nylon 66 biotrans membranes
KW - PhastGel
KW - Polyacrylamide gel
KW - Protein blotting
KW - Ultrasound
UR - http://www.scopus.com/inward/record.url?scp=84930959007&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-2694-7_25
DO - 10.1007/978-1-4939-2694-7_25
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 26044006
AN - SCOPUS:84958623787
SN - 1064-3745
VL - 1312
SP - 237
EP - 246
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -