TY - JOUR
T1 - A simple and rapid HPLC method for the detection of glyburide in plasma original research communication (analytical)
AU - Gedeon, Christelle
AU - Kapur, Bhushan
AU - Aleksa, Katarina
AU - Koren, Gideon
N1 - Funding Information:
Research supported by a grant from CIHR. C. Gedeon is supported by the Restracomp studentship from the Hospital for Sick Children. G. Koren holds the Ivey Chair in Molecular Toxicology at the University of Western Ontario.
PY - 2008/2
Y1 - 2008/2
N2 - Introduction: Although a variety of HPLC methods have been previously described for the quantification of glyburide, attempts to implement them clinically have been unsuccessful. Some are time consuming, expensive, or not directly applicable to human plasma. Others are outdated, as the necessary materials are no longer available. Objective: To describe a simple, rapid, and sensitive HPLC method for the analysis of human plasma and perfusate. Design and method: Samples were extracted by liquid-liquid extraction with chloroform at neutral pH. Glyburide was detected at 254 nm, with a total run time of 7 min per sample. Results: Standard curves of 50 to 400 ng/mL of glyburide were linear (r2 = 0.998). Inter-day and intra-day sample coefficient of variations were 8% and 4%, respectively. Recoveries ranged from 71 to 75% in human plasma samples for the 20-400 ng/mL concentration range. Conclusion: Clinically, this method can be applied in the therapeutic drug monitoring of glyburide.
AB - Introduction: Although a variety of HPLC methods have been previously described for the quantification of glyburide, attempts to implement them clinically have been unsuccessful. Some are time consuming, expensive, or not directly applicable to human plasma. Others are outdated, as the necessary materials are no longer available. Objective: To describe a simple, rapid, and sensitive HPLC method for the analysis of human plasma and perfusate. Design and method: Samples were extracted by liquid-liquid extraction with chloroform at neutral pH. Glyburide was detected at 254 nm, with a total run time of 7 min per sample. Results: Standard curves of 50 to 400 ng/mL of glyburide were linear (r2 = 0.998). Inter-day and intra-day sample coefficient of variations were 8% and 4%, respectively. Recoveries ranged from 71 to 75% in human plasma samples for the 20-400 ng/mL concentration range. Conclusion: Clinically, this method can be applied in the therapeutic drug monitoring of glyburide.
KW - Clinical
KW - Glyburide
KW - HPLC
KW - Human Plasma
KW - Liquid-liquid extraction
KW - UV Detection
UR - https://www.scopus.com/pages/publications/38649127226
U2 - 10.1016/j.clinbiochem.2007.07.025
DO - 10.1016/j.clinbiochem.2007.07.025
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C2 - 18061587
AN - SCOPUS:38649127226
SN - 0009-9120
VL - 41
SP - 167
EP - 173
JO - Clinical Biochemistry
JF - Clinical Biochemistry
IS - 3
ER -