A general approach for antibody purification utilizing [Protein A-Catechol:Fe3+] macro-complexes

Guy Patchornik; Amnon Albeck

doi:10.1021/bc050361+

A general approach for antibody purification utilizing [Protein A-Catechol:Fe³⁺] macro-complexes

Guy Patchornik
, Amnon Albeck

نتاج البحث: نشر في مجلة › مقالة › مراجعة النظراء

4 اقتباسات (Scopus)

ملخص

A general platform for antibody purification utilizing free nonimmobilized Protein A modified with the strong metal chelator catechol (ProA-CAT) and Fe³⁺ ions is presented. The mechanism of purification requires formation and precipitation of macro-complexes composed of [ProA-CAT:IgG:Fe ³⁺]. Target IgGs are eluted directly from the precipitates (i.e. pellets) at pH 3 in high yields (71-80%) and high purity (>95%), without dissociating the [ProA-CAT:Fe³⁺] insoluble macro-complex.

اللغة الأصلية	الإنجليزيّة
الصفحات (من إلى)	258-260
عدد الصفحات	3
دورية	Bioconjugate Chemistry
مستوى الصوت	17
رقم الإصدار	2
المعرِّفات الرقمية للأشياء	https://doi.org/10.1021/bc050361+
حالة النشر	نُشِر - 2006
منشور خارجيًا	نعم

الوصول إلى المستند

10.1021/bc050361+

الملفات والروابط الأخرى

Link to publication in Scopus

قم بذكر هذا

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title = "A general approach for antibody purification utilizing [Protein A-Catechol:Fe3+] macro-complexes",

abstract = "A general platform for antibody purification utilizing free nonimmobilized Protein A modified with the strong metal chelator catechol (ProA-CAT) and Fe3+ ions is presented. The mechanism of purification requires formation and precipitation of macro-complexes composed of [ProA-CAT:IgG:Fe 3+]. Target IgGs are eluted directly from the precipitates (i.e. pellets) at pH 3 in high yields (71-80\%) and high purity (>95\%), without dissociating the [ProA-CAT:Fe3+] insoluble macro-complex.",

author = "Guy Patchornik and Amnon Albeck",

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doi = "10.1021/bc050361+",

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AB - A general platform for antibody purification utilizing free nonimmobilized Protein A modified with the strong metal chelator catechol (ProA-CAT) and Fe3+ ions is presented. The mechanism of purification requires formation and precipitation of macro-complexes composed of [ProA-CAT:IgG:Fe 3+]. Target IgGs are eluted directly from the precipitates (i.e. pellets) at pH 3 in high yields (71-80%) and high purity (>95%), without dissociating the [ProA-CAT:Fe3+] insoluble macro-complex.

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